subtracting cdna library meaning in English
递减式cdna文库
Examples
- Seven independent ests were obtained from the forward subtracted cdna library
结果正向差减文库中获得7个独立的est 。 - Finally , the forward and backward subtracted cdna libraries including 863 and 360 clones respectively were obtained
最后所获正向差减文库含863个重组子，反向差减文库含360个重组子。 - Chapter 2 screening of genes related to the ovary development of the mitten crab ( eriocheir japonica sinensis ) by cdna macroarray analysis plasmids were extracted from all the clones in the subtracted cdna libraries with alkali lysis method , then the inserted fragments in plasmids were amplified by pcr
2用cdna微阵列筛选中华绒螯蟹卵巢发育相关基因将差减cdna文库中的所有克隆用碱裂解法提取质粒，然后以提取的质粒为模板，用pcr方法扩增插入片段。 - The length of the inserted fragments of the forward and backward subtracted cdna libraries was 360 and 160 base pairs in average respectively by pcr detection . the results showed that the subtracted libraries constructed were suitable for further study on the genes related to the ovary development of the mitten crab
Pcr扩增鉴定正、反向差减cdna文库的插入片段平均大小分别为360bp和160bp ，表明所构建的差减文库适合进一步研究中华绒螯蟹卵巢发育相关基因。 - Part 1 screening of genes related to the ovary development of the mitten crab ( eriocheir japonica sinensis ) chapter 1 construction of subtractive cdna library of mitten crab ( eriocheir japonica sinensis ) ovary two subtracted cdna libraries of mitten crab ( eriocheir japonica sinensis ) ovaries at two successive developmental stages were constructed by suppression subtractive hybridization ( ssh )
第一部分中华绒螯蟹卵巢发育相关基因的筛选1中华绒螯蟹卵巢差减cdna文库的构建应用抑制性差减杂交技术，构建了中华绒螯蟹卵巢两个发育时期的差减cdna文库。